Human Anti-EPHA2 Recombinant Antibody (clone 4H5)
CAT#: HPAB-0453-CN
4H5 is an antibody which is humanized from murine antibody EA2. 4H5 exhibits approximately a 20 fold increase of affinity when compared with chimeric Fab EA2.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles, plasma membrane, the Golgi apparatus & cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10464/991_D3_3_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (CAPAN-2, PC-3, U-251 MG, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142627-EPHA2
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SDS-PAGE
Figure 1 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in SDS-PAGE
SDS-PAGE analysis of HPAB-0453-CN in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in SEC-HPLC
The purity of HPAB-0453-CN was 99.1491% as determined by SEC-HPLCInjection: 10 µlInjection: 10 µl
WB
Figure 3 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in WB
Western blot analysis of HPAB-0453-CN was performed by loading EPHA2 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0453-CN and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
DB
Figure 4 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in DB
Dot Blot analysis of HPAB-0453-CN was performed by coating with EPHA2 protein (His tag).
HPAB-0453-CN incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 5 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in ELISA
ELISA analysis of HPAB-0453-CN was performed by coating with EPHA2 protein (His tag). Then blocked with BSA and incubated with HPAB-0453-CN. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human EPHA2
- Species Reactivity
- Human
- Clone
- 4H5
- Applications
- ELISA, FC, IF
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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(Creative Biolabs Cat# HPAB-0453-CN, RRID: AB_3111398)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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