Human Anti-EPHA2 Recombinant Antibody (clone 4H5) (CAT#: HPAB-0453-CN)
4H5 is an antibody which is humanized from murine antibody EA2. 4H5 exhibits approximately a 20 fold increase of affinity when compared with chimeric Fab EA2.
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SDS-PAGE
Figure 1 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in SDS-PAGE
SDS-PAGE analysis of HPAB-0453-CN in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in SEC-HPLC
The purity of HPAB-0453-CN was 99.1491% as determined by SEC-HPLCInjection: 10 µlInjection: 10 µl
WB
Figure 3 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in WB
Western blot analysis of HPAB-0453-CN was performed by loading EPHA2 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0453-CN and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
DB
Figure 4 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in DB
Dot Blot analysis of HPAB-0453-CN was performed by coating with EPHA2 protein (His tag).
HPAB-0453-CN incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 5 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in ELISA
ELISA analysis of HPAB-0453-CN was performed by coating with EPHA2 protein (His tag). Then blocked with BSA and incubated with HPAB-0453-CN. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human EPHA2
- Species Reactivity
- Human
- Clone
- 4H5
- Applications
- ELISA, FC, IF
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- EPH Receptor A2; Tyrosine-Protein Kinase Receptor ECK; EC 2.7.10.1; ECK; Epithelial Cell Receptor Protein Tyrosine Kinase; Soluble EPHA2 Variant 1; Epithelial Cell Kinase; EC 2.7.10; CTRCT6; ARCC2; CTPP1; EphA2; CTPA
- Gene ID
- 1969
- UniProt ID
- P29317
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-385MZ | Human Anti-EPHA2 Recombinant Antibody (TAB-385MZ) | ELISA | Humanized IgG |
| TAB-385MZ-S(P) | Human Anti-EPHA2 Recombinant Antibody; scFv Fragment (TAB-385MZ-S(P)) | ELISA | Humanized scFv |
| TAB-385MZ-F(E) | Human Anti-EPHA2 Recombinant Antibody; Fab Fragment (TAB-385MZ-F(E)) | ELISA | Humanized Fab |
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0453-CN, RRID: AB_3111398)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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